RNA-cleaving Fluorescent DNAzymes (RFDs)
RFDs are DNA sequences that induce a cleavage (cutting) reaction in a substrate strand in response to the presence of a target. In this project, we at saiwa team segmented and measured regions of interest (the dots that constitute the microarray) relative to the background in printed RFD images. Printed microarrays can be used to test for the presence of specific targets (i.e., bacteria). We used around 100 RFD images that were provided by Didar Lab., McMaster uni.
- Timeline
- Nov 2021 – Jan 2022
- Platform
- Country
- Technology
Problem
The detection and analysis of specific biological targets, such as bacteria, are critical in various fields, including medical diagnostics and environmental monitoring. Traditional methods of target detection can be time-consuming, labor-intensive, and require complex equipment, making them less accessible for rapid and on-site testing.
Solution
Saiwa has collaborated with McMaster University to develop a novel approach using RNA-cleaving Fluorescent DNAzymes (RFDs). These are specialized DNA sequences that respond to the presence of a target by inducing a cleavage reaction in a substrate strand. Our team has utilized this technology to create printed microarrays that segment and measure regions of interest (the dots on the microarray) against the background in RFD images. This method allows for the precise detection of specific targets through visual analysis of around 100 RFD images provided by Didar Lab at McMaster University.
Outcome
The project has successfully streamlined the process of target detection. The printed microarrays offer a quick, efficient, and accurate method for identifying specific biological entities. This advancement has significant implications for improving the speed and accuracy of diagnostics and environmental assessments, thereby enhancing the ability to respond to biological threats and monitor health conditions more effectively.
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